Effects of Liquid Medium Environment on Elastic Modulus of Breast Cancer Cells
10.16156/j.1004-7220.2021.03.24
- VernacularTitle:液态介质环境对乳腺癌细胞弹性模量的影响
- Author:
Weihao SUN
1
;
Jianli MA
1
;
Jie YU
1
;
Chengwei WU
1
;
Wei ZHANG
1
Author Information
1. State Key Laboratory of Structural Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology
- Publication Type:Journal Article
- Keywords:
breast cancer cells;
elastic modulus;
adhesion force;
liquid medium environment
- From:
Journal of Medical Biomechanics
2021;36(3):E448-E452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of three kinds of commonly used liquid culture media for in vitro cell experiments on elastic modulus of breast cancer cells, so as to provide references for developing novel diagnosis and treatment approach of tumour based on mechanics principles. Methods The elastic modulus and adhesion force of breast cancer cells MCF7 to atomic force microscopy (AFM) probes in phosphate buffered solution(PBS), Dulbecco’s modified eagle media (DMEM) and DMEM+10% fetal bovine serum (FBS) were measured using AFM technology. Results The elastic moduli of breast cancer cells in PBS, DMEM and DMEM+10% FBS were 2.59, 2.11 and 1.59 kPa, respectively. The cell adhesion forces in the above three kinds of liquid medium environment were 63.81, 66.09 and 121.97 pN, respectively. Cell adhesion force in DMEM+10%FBS was significantly different from that of the other two kinds of liquid media. Conclusions There are significant differences in elastic modulus of breast cancer cells in three kinds of liquid media. The difference between DMEM and DMEM+10%FBS might be caused by the different adhesion force caused by serum proteins in the media, and the difference between DMEM and PBS might be attributed to the difference in pH of the media.
