Development of Acellular Respiratory Mucosal Matrix Using Porcine Tracheal Mucosa
10.1007/s13770-020-00260-w
- Author:
Soo Yeon JUNG
1
;
An Nguyen-Thuy TRAN
;
Ha Yeong KIM
;
Euno CHOI
;
So Jeong LEE
;
Han Su KIM
Author Information
1. Department of Otorhinolaryngology - Head and Neck Surgery, College of Medicine, Ewha Womans University, Anyangcheon-ro 1071, Yang Cheon-Gu, Seoul 07985, Korea
- Publication Type:IGINAL ARTICLE
- From:
Tissue Engineering and Regenerative Medicine
2020;17(4):433-443
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND:Respiratory mucosa defects result in airway obstruction and infection, requiring subsequent functionalrecovery of the respiratory epithelium. Because site-specific extracellular matrix (ECM) facilitates restoration of organfunction by promoting cellular migration and engraftment, previous studies considered decellularized trachea an idealECM; however, incomplete cell removal from cartilage and mucosal-architecture destruction are frequently reported. Here,we developed a decellularization protocol and applied it to the respiratory mucosa of separated porcine tracheas.
METHODS:The trachea was divided into groups according to decellularization protocol: native mucosa, freezing–thawing (FT), FT followed by the use of Perasafe-based chemical agents before mucosal separation (wFTP), after mucosalseparation (mFTP), and followed by DNase decellularization (mFTD). Decellularization efficacy was evaluated by DNAquantification and hematoxylin and eosin staining, and ECM content of the scaffold was evaluated by histologic analysisand glycosaminoglycan and collagen assays. Biocompatibility was assessed by cell-viability assay and in vivotransplantation.
RESULTS:The mFTP mucosa showed low antigenicity and maintained the ECM to form a proper microstructure.Additionally, tonsil-derived stem cells remained viable when cultured with or seeded onto mFTP mucosa, and the in vivohost response showed a constructive pattern following implantation of the mFTP scaffolds.
CONCLUSION:These results demonstrated that xenogenic acellular respiratory mucosa matrix displayed suitable biocompatibilityas a scaffold material for respiratory mucosa engineering.
