Development of Calcification-Resistant Bovine Pericardium with PEO-SO3 (I): An implantation study of bovine pericardium at aorta and pulmonary artery in canine model.
- Author:
Hyoung Mook KIM
1
;
Hak Jae KIM
;
In Sung LEE
;
Kwang Taik KIM
;
Kyung SUN
;
Man Jong BAEK
;
Won Gyu LEE
;
Gee Dong PARK
Author Information
1. Department of Thoracic & Cardiovascular Surgery, Korea University, College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Calcinosis;
Pericardial patch
- MeSH:
Adult;
Animals;
Aorta*;
Aorta, Thoracic;
Calcinosis;
Calcium;
Dogs;
Follow-Up Studies;
Glutaral;
Heterografts;
Hominidae;
Human Body;
Humans;
Hydrogen-Ion Concentration;
Pericardium*;
Polyethylene Glycols;
Prostheses and Implants;
Pulmonary Artery*
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
1998;31(10):919-923
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Calcific degeneration is unavoidable in either homo or heterografts implanted in the human body. We have developed a calcification-resistant cardiovascular tissue patch using a novel technique of anticalcification. MATERIALS AND METHODS: Fresh bovine pericardium was harvested at the slaughter house and transfered to the laboratory in Hank's solution. After trimming and fixing the pericardium, it was embedded in 4degree C 0.65% glutaraldehyde for a week and then washed by phosphate-buffered saline (PBS) of pH 7.4. This prepared pericardium was then stored in 2.5% sulphonated polyethyleneoxide (PEO-SO3) solution for 2 days at room temperature and reversed by 4degree C NaBH4 solution for 16 hours. To evaluate the calcification-resistance of surface modified bovine pericardium with PEO-SO3, either glutaraldehyde-treated (GA group, n=4) or PEO-SO3-treated pericardial patch (PEO-SO3 group, n=4) was implanted into adult mongrel dog to reconstruct the main pulmonary artery and the descending aorta using a partial clamp technique. After 1 month follow-up, the implanted patches were retrieved to evaluate the pathologic findings and the content of calcium and phosphorous. RESULTS: The PEO-SO3 group showed substantially less retraction and significantly less calcium deposition than the GA group in both aortic (7.10+/-1.05 vs. 13.81+/-2.33 mg/g of dried tissue) and pulmonary positions (1.55+/-0.29 vs. 6.72+/-0.70 mg/g) (p<0.01). Phosphorous contents were also less in the PEO-SO3 group than the GA group significantly, 8.11+/-1.07 mg/g vs. 19.33+/-4.31 mg/g in the aortic and 2.58+/-0.40 vs. 12.60+/-3.40 mg/g in thepulmonary position (p<0.01). CONCLUSIONS: These findings suggest that PEO-SO3 modified bovine pericardium is highly calcification-resistant but further study is needed to evaluate the long-term biological safety and compatibility of the prosthesis.
