The Effect of Freezing-thawing Activated Platelet Rich Plasmas (PRP) on the Proliferations of Bacteria.
- Author:
Young Ae LIM
1
;
SaeYun BAIK
;
Wee Gyo LEE
Author Information
1. Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea. limyoung@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Platelet rich plasma (PRP);
Antibacterial effect;
Freezing-thawing
- MeSH:
Adenine;
Bacteria;
Blood Platelets;
Citrates;
Diffusion;
Glucose;
Heparin;
Humans;
Phosphates;
Plasma;
Platelet Count;
Platelet-Rich Plasma;
Thrombin;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2011;22(3):221-230
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Fresh platelet rich plasma (PRP) gel has been reported to have anti-bacterial properties. We evaluated the anti-bacterial effects of liquid type activated PRP (tAPRP) using thrombin and heparin treatment after a freezing-thawing (F-T) procedure, using a disk diffusion method. METHODS: PRP and platelet poor plasma (PPP) were prepared from CPDA-1 anticoagulated blood received from 20 donors. PRP was concentrated to 8 times the base platelet counts of donors for the first trial and to 11 times the base platelet counts of donors for the second trial. Both F-T PRP and F-T PPP were divided into a nonactivated group and an activated (tA) group, which was then treated with bovine thrombin and CaCl2, and heparin was added to prevent gel formation. The anti-bacterial effects of F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin on S. aureus and P. aeruginosa were evaluated using a disk-diffusion and direct dropping method. All experiments were duplicated. RESULTS: The inhibited diameters resulting for S. aureus and P. aeruginosa, using the disk-diffusion and direct dropping method, were zero for all 20 sets of results for F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin. CONCLUSION: No anti-bacterial effects were detected for S. aureus or P. aeruginosa in the F-T PRP, F-T PPP, F-T tAPRP with heparin and F-T tAPPP with heparin. This negative result may be due to the F-T treatment and/or because liquid instead of gel form of PRP was used. The use of the disk diffusion method for the determination of anti-bacterial ability of PRP may also be a factor in the negative study results.
