Expression of Bcl-2 Protein in Ischemia-Reperfused Myocardium of Rabbit.
- Author:
Sam Hyun KIM
1
;
Yee Tae PARK
;
Pil Won SEO
;
Jae Wook RYU
;
Sung Sik PARK
;
Young Kwon KIM
;
Chang Hyu CHOI
;
Gyung Min RYU
;
Sung Sook KIM
Author Information
1. Department of Cardiothoracic Surgery, College of Medicine, Dankook University, Cheonan, Korea.
- Publication Type:Original Article
- Keywords:
Myocardial reperfusion;
Myocardial protection
- MeSH:
Animals;
Apoptosis;
Cell Death;
Coronary Vessels;
Cytoplasm;
Formaldehyde;
Heart;
Muscle Cells;
Myocardial Infarction;
Myocardial Reperfusion;
Myocardium*;
Necrosis;
Paraffin;
Rabbits;
Reperfusion
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
1998;31(10):924-927
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Myocardial cell death after myocardial infarction or reperfusion is classified into necrosis and apoptosis. Bcl-2 protein is a cytoplasmic protein, which inhibits apoptosis and is expressed in acute stage of myocardial infarction but not in normal heart. This study was performed to investigate whether Bcl-2 protein was expressed respectively to the reperfusion time. MATERIALS AND METHODS: Thirty nine New Zealand white rabbits weighing 1.5-4.8 kg (mean, 2.9kg) were alloted into 7 groups (n=5 in each group) which underwent left anterior descending coronary artery (LAD) occlusion for 30 minutes, followed by reperfusion. The animals were sacrificed at 1, 4, 8, 12, 24 hours, and 3, 7 days after occlusion. Ventricle was excised immediately after intervention. Tissues were fixed in 10% buffured formalin and embedded in paraffin. Bcl-2 protein was detected by immunohistochemical stain with using monoclonal antibody against Bcl-2 protein. RESULTS: The positive immunohistochemical reactivity for Bcl-2 protein was observed in 12, 24 hours, and 3 days reperfusion groups. Bcl-2 protein was detected in salvaged myocytes surrounding the infarcted area. CONCLUSIONS: Bcl-2 protein is expressed at the late acute stage of infarct. Therefore, the expression of Bcl-2 protein may not protect acute cell death, but may play a role in the prevention of late cell death after myocardial is chemia-reperfusion.
