The Blocking of c-Met Signaling Induces Apoptosis through the Increase of p53 Protein in Lung Cancer.
- Author:
Hae Yun JUNG
1
;
Hyun Jung JOO
;
Jong Kuk PARK
;
Yeul Hong KIM
Author Information
1. Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea. yhk0215@korea.ac.kr
- Publication Type:Original Article
- Keywords:
((3Z)-N-(3-chlorophenyl)-3-((3,5-dimethyl-4-((4-methylpiperazin-1-yl)carbonyl)-1H-pyrrol-2-yl)methylene)-N-methyl-2-oxo-2;
3-dihydro-1H-indole-5-sulfonamide);
Tumor suppressor protein p53;
Apoptosis;
Lung neoplasms;
Molecular targeted therapy
- MeSH:
Apoptosis;
Blotting, Western;
Caspase 3;
Down-Regulation;
Flow Cytometry;
Growth Inhibitors;
Humans;
Indoles;
Lung;
Lung Neoplasms;
Molecular Targeted Therapy;
Piperazines;
Protein-Tyrosine Kinases;
Puma;
Sulfonamides;
Transplantation, Heterologous;
Tumor Burden;
Tumor Suppressor Protein p53;
Up-Regulation
- From:Cancer Research and Treatment
2012;44(4):251-261
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: c-Met is an attractive potential target for novel therapeutic inhibition of human cancer, and c-Met tyrosine kinase inhibitors (TKIs) are effective growth inhibitors of various malignancies. However, their mechanisms in anticancer effects are not clear. In the present study, we investigated the possibility that blocking c-Met signaling induces p53-mediated growth inhibition in lung cancer. MATERIALS AND METHODS: The growth inhibitory effects of c-Met TKI (SU11274) on lung cancer cells and a xenograft model were assessed using the MTT assay, flow cytometry, and terminal deoxyribonucleotide transferase-mediated nick-end labeling staining. The role of p53 protein in the sensitivity of c-Met TKI (SU11274) was examined by Western blot analysis and immunohistochemistry. RESULTS: SU11274 significantly induced apoptosis in A549 cells with wild-type p53, compared with that in Calu-1 cells with null-type p53. SU11274 increased p53 protein by enhancing the stability of p53 protein. Increased p53 protein by SU11274 induced up-regulation of Bax and PUMA expression and down-regulation of Bcl-2 expression, subsequently activating caspase 3. In p53 knock-out and knock-in systems, we confirmed that SU11274 caused apoptosis through the p53-mediated apoptotic pathway. Likewise, in the A549 xenograft model, SU11274 effectively shrank tumor volume and induced apoptosis via increased p53 protein expression. Blocking c-Met signaling increased the level of p53 protein. CONCLUSION: Our finding suggested that p53 plays an important role in SU11274-induced apoptosis, and p53 status seems to be related to the sensitivity to SU11274 in lung cancer.
