Identification of a mimotope of an infectious bronchitis virus S1 protein
- Author:
Jingming ZHOU
1
;
Jianan LI
;
Yanghui LI
;
Hongliang LIU
;
Yanhua QI
;
Aiping WANG
Author Information
- Publication Type:Rapid Communication
- From:Journal of Veterinary Science 2021;22(4):e49-
- CountryRepublic of Korea
- Language:English
- Abstract: The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzymelinked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.
