Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip
- Author:
Jung Min KIM
1
;
Su Jin LIM
;
SoMin KIM
;
MoonJung KIM
;
ByoungHee KIM
;
Truong A TAI
;
Seonmi KIM
;
ByoungSu YOON
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2020;21(1):e4-
- CountryRepublic of Korea
- Language:English
- Abstract: Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.
