Erythropoietin (EPO) Attenuates Renal Injury in an Experimental Model of Cisplatin-induced Nephrotoxicity in Rats.
- Author:
Dae Eun CHOI
1
;
Sarah CHUNG
;
Young Mo LEE
;
Kwang Sun SUH
;
Ki Ryang NA
;
Young Tai SHIN
;
Kang Wook LEE
Author Information
1. Department of Internal Medicine, Chungnam National University Hospital, Chungnam, Korea. kwlee@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Cisplatin;
Erythropoietin;
Inflammation
- MeSH:
Acute Kidney Injury;
Animals;
Apoptosis;
Cisplatin;
Creatinine;
Erythropoietin;
Inflammation;
Models, Animal;
Models, Theoretical;
Rats;
RNA, Messenger;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Nephrology
2009;28(2):103-112
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE:In addition to its hematopoietic effects, EPO has protective effects in vivo in several animal models of acute renal injury. We examined whether EPO also attenuated renal injury in a rat model of cisplatin-induced nephrotoxicity via anti-apoptotic and anti-inflammatory actions. METHODS:Male SpragueDawley rats were divided into four groups: control rats, EPO+control rats, cisplatin rats, and EPO+cisplatin rats. EPO treatment was started 24 h prior to cisplatin administration. Then, 96 h after cisplatin administration, all experimental animals were killed. And renal molecular, functional and structural parameters were measured. RESULTS:The serum levels of BUN and creatinine in the 96 h after cisplatin administration were significantly lower than in cisplatin rats. On microscopic examination, the magnitude of renal tubular epithelial damage in the EPO+cisplatin rats was also significantly less than that of cisplatin rats. Renal expression of TNF-alpha Fas, MCP-1 and TGF-betain the cisplatin rats was significantly higher than those of control rats and EPO+control rats. The levels of TNF-alpha Fas, MCP-1 and TGF-betagene expression in EPO+cisplatin rats were significantly lower than those of cisplatin rats. The Bcl-2 mRNA level in EPO+cisplatin rats was significantly higher than in cisplatin rats. EPO+cisplatin rats had significantly fewer TUNEL-positive cells. CONCLUSION:These results suggest that EPO has a protective effect against experimental cisplatin- induced renal injury and that the anti-inflammatory and anti-apoptotic properties of EPO may be involved.
