Performance of the PROTIA(TM) Allergy-Q(R) System in the Detection of Allergen-specific IgE: A Comparison With the ImmunoCAP(R) System.
10.4168/aair.2015.7.6.565
- Author:
Jae Hyun LEE
1
;
Hye Jung PARK
;
Kyung Hee PARK
;
Kyoung Yong JEONG
;
Jung Won PARK
Author Information
1. Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. parkjw@yuhs.ac
- Publication Type:Original Article
- Keywords:
Allergen;
specific IgE;
detection performance;
allergic disease;
Allergy-Q(R);
ImmunoCAP(R)
- MeSH:
Allergens;
Alternaria;
Artemisia;
Asthma;
Dermatitis, Atopic;
Food Hypersensitivity;
Humans;
Hypersensitivity;
Immunoenzyme Techniques;
Immunoglobulin E*;
Immunoglobulins;
Mass Screening;
Rhinitis
- From:Allergy, Asthma & Immunology Research
2015;7(6):565-572
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The PROTIA(TM) Allergy-Q(R) enzyme immunoassay (EIA) is a recently developed screening assay for specific immunoglobulin E (sIgE) for multiple allergens. The ImmunoCAP(R) fluorescent EIA (FEIA) system is the most widely used method for sIgE detection. In this study, we evaluated the performance of the Allergy-Q(R) system compared to the ImmunoCAP(R) system. METHODS: We compared the 2 systems using sera from 260 Korean allergy patients suffering from asthma (26.5%), allergic rhinitis (42.3%), atopic dermatitis (67.7%), and food allergy (18.1%). We compared sIgE-measurement results for 7 inhalant allergens, 5 food allergens, and 4 microorganism allergens. RESULTS: Overall, 1,799 paired assay results were analyzed. Except mugwort and alternaria, most of the allergen-sIgE results showed intra-class correlation coefficients of >0.5. Inter-assay class associations were reliable for most allergens (gamma=0.858-0.987, P<0.001). Passing-Bablok regression analysis showed multiple differences in intercept and slope. The inter-method concordance was moderate to substantial for most allergens (kappa=0.713-0.898, P<0.001). CONCLUSIONS: The PROTIA(TM) Allergy-Q(R) EIA system exhibited good detection performance compared to the ImmunoCAP(R) FEIA system in Korean allergic patients. However, because of methodological differences between the 2 assays, careful clinical implication is required for the interpretation of Allergy-Q(R) EIA results.
