Alpha-lipoic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced cell injury by inhibiting autophagy and apoptosis
10.11620/IJOB.2021.46.1.15
- Author:
Kyeong-Rok KANG
1
;
Jae-Sung KIM
;
Tae-Hyeon KIM
;
Jeong-Yeon SEO
;
HyangI LIM
;
Jong-Hyun PARK
;
Kwang Yeol YANG
;
Sun-Kyoung YU
;
Heung-Joong KIM
;
Chun Sung KIM
;
Hong Sung CHUN
;
Dong-Seol LEE
;
Joo-Cheol PARK
;
Do Kyung KIM
Author Information
1. The Institute of Dental Science, Chosun University, Gwangju 61452, Republic of Korea
- Publication Type:Original Article
- From:International Journal of Oral Biology
2021;46(1):15-22
- CountryRepublic of Korea
- Language:English
-
Abstract:
Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and BclxL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.
