Effect of Ultraviolet Light on the Expression of Adhesion Molecules and T Lymphocyte Adhesion to Human Dermal Microvascular Endothelial Cells.
10.3349/ymj.2002.43.2.165
- Author:
Kee Yang CHUNG
1
;
Nam Soo CHANG
;
Yoon Kee PARK
;
Kwang Hoon LEE
Author Information
1. Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea. kwanglee@yumc.yonsei.ac.kr
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
HDMEC;
ultraviolet radiation;
adhesion molecules;
intercellular adhesion molecule-1;
E-selectin;
vascular cell adhesion molecule-1
- MeSH:
Cell Adhesion/radiation effects;
Cell Adhesion Molecules/*metabolism;
Cells, Cultured;
Endothelium, Vascular/cytology/*physiology;
Human;
Microcirculation;
Skin/*blood supply;
T-Lymphocytes/*physiology;
*Ultraviolet Rays
- From:Yonsei Medical Journal
2002;43(2):165-174
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to determine the effect of ultraviolet radiation (UVR) on the cell adhesion molecules expressed in human dermal microvascular endothelial cells (HDMEC), the cells were exposed to varying UVR doses and the cell surface was examined for expression of intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM- 1), and E-selectin. The effect of UVB irradiation on the binding of T lymphocytes to HDMEC was also examined. UVA irradiation did not affect the surface expression of ICAM-1, VCAM-1, or E-selectin on the HDMEC. However, following UVB exposure, ELISA demonstrated a significant increase in the baseline ICAM-1 cell surface expression on the HDMEC. However, no induction of either E-selectin or VCAM-1 was noted. UVB also significantly augmented ICAM-1 induction by IL-1 alpha and TNF- alpha. VCAM-1 was induced by stimulating HDMEC with IL-1alpha following a UVB irradiation dose of 100 mJ/cm2. Flow cytometric analysis of the HDMEC stimulated with IL-1 alpha for 24h demonstrated that 12% of the cells expressed VCAM-1 but either IL-1 alpha or UVB irradiation alone failed to induce VCAM-1 expression. Enhancement of T cell-HDMEC binding by IL-1 alpha; or TNF- alpha treatment was not significantly affected after UVB irradiation. This study demonstrated that UVB irradiation can alter ICAM-1 and VCAM-1 expression on the HDMEC surface and that augmentation of ICAM-1 expression and the IL-1 alpha -dependent induction of VCAM-1 following UVB exposure might be important steps in the pathogenesis of sunburn.
