The Combination of Concentrated Growth Factor and AdiposeDerived Stem Cell Sheet Repairs Skull Defects in Rats
10.1007/s13770-021-00371-y
- Author:
Tuqiang HU
1
;
Hao ZHANG
;
Wei YU
;
Xuezhou YU
;
Zubing LI
;
Li HE
Author Information
1. Department of Stomatology, Renmin Hospital, Hubei University of Medicine, NO.39 Chaoyang Middle Road, Maojian District, Shiyan 442000, Hubei, China
- Publication Type:ORIGINAL ARTICLE
- From:
Tissue Engineering and Regenerative Medicine
2021;18(5):905-913
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND:The goal of this study was to create a biomaterial which combines concentrated growth factor (CGF) with an adipose-derived stem cell (ADSC) sheet to promote the repair of skull defects in rats.
METHODS:We determined the optimal concentration of CGF extract by investigating the effects of different concentrations (0, 5%, 10%, and 20%) on the proliferation and differentiation of ADSCs. Then we created a complex combining CGF with an ADSC sheet, and tested the effects on bone repair in four experimental rat groups: (A) control; (B) ADSC sheet; (C) CGF particles; (D) combination of CGF ? ADSCs. Eight weeks after the procedure, osteogenesis was assessed by micro-CT and hematoxylin and eosin staining.
RESULTS:We found that the concentration of CGF extract that promoted optimal ADSC proliferation and differentiation in vitro was 20%. In turn, bone regeneration was promoted the most by the combination of CGF and ADSCs.
CONCLUSION:In this study, we determined the optimal ratio of CGF and ADSCs to be used in a biomaterial for bone regeneration. The resulting CGF/ADSCs complex promotes maxillofacial bone defect repair in rats.
