Genetic Manipulation and Transformation Methods for Aspergillus spp.
10.1080/12298093.2020.1838115
- Author:
Ye-Eun SON
1
;
Hee-Soo PARK
Author Information
1. School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea
- Publication Type:REVIEW ARTICLE
- From:Mycobiology
2021;49(2):95-104
- CountryRepublic of Korea
- Language:English
-
Abstract:
Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus Aspergillus. The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.
