Construction of an avian hepatitis E virus replicon expressing heterologous genes and evaluation of its potential as an RNA vaccine platform
10.14405/kjvr.2021.61.e11
- Author:
Hyun-Woo MOON
1
;
Haan Woo SUNG
;
Jeongho PARK
;
Hyuk Moo KWON
Author Information
1. Department of Veterinary Microbiology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea
- Publication Type:Short Communication
- From:Korean Journal of Veterinary Research
2021;61(2):e11-
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate avian hepatitis E virus (aHEV) as an RNA vaccine platform, open reading frame 2 (ORF2) of aHEV was replaced by heterologous genes, such as enhanced green fluorescent protein (eGFP) and hemagglutinin (HA)-tag, in aHEV infectious cDNA clones. eGFP and HA-tag replicons were expressed in leghorn male hepatoma (LMH) cells. To confirm expression of the heterologous protein, ORF2 was replaced with the antigenic S1 gene of infectious bronchitis virus (IBV). The IBVS1 replicon was expressed in LMH cells. To our knowledge, this is the first investigation showing potential as a RNA vaccine platform using an aHEV. In the future, it may be used in the development of RNA vaccines against various pathogens.
