Evaluation of cytotoxicity and inflammatory tissue response of mineral trioxide aggregates containing dicalcium phosphate dihydrate

Author: Hyun Jae PARK ¹ ; Hee Chul PARK ; Gitae SON ; Yongjoon KIM ; Lele WU ; Sang Hoon CHOI ; Hyeong-Cheol YANG
Author Information

1. Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea
Publication Type:Original Article
From: Korean Journal of Dental Materials 2021;48(2):125-134
CountryRepublic of Korea
Language:English
Abstract: The purpose of this study was to evaluate the effect of dicalcium phosphate dihydrate (DCPD) on the biocompatibility of mineral trioxide aggregate (MTA). DCPD was added to MTA (OrthoMTA) to suppress the increase in pH of MTA during hardening, and the change of pH, cytotoxicity, and subcutaneous inflammation reactions in mouse model were observed. The pH of OrthoMTA and DCPD-OrthoMTA at 1st day in phosphate-buffered saline was 12.5 and 12.8, respectively. At 19th day, the pH was 11.6 (OrthoMTA) and 8.8 (DCPD-OrthoMTA). Cytotoxicity of DCPD-OrthoMTA extract was lesser than that of OrthoMTA at high concentration (above 50%) (p<0.05). No significant differences appeared in subcutaneous inflammatory reactions among ProRoot MTA, OrthoMTA and DCPD-OrthoMTA. Therefore, it is likely that there is no apparent relationship between the cytotoxicity and subcutaneous inflammation in our experimental conditions.