Studies on the bfp Gene, Adherence to HEp-2 Cells and Serotyping of Escherichia coli Isolated from Urine.
- Author:
Yung Bu KIM
;
Min Jung KIM
;
Geun Sam PARK
- Publication Type:Original Article
- MeSH:
Blood Cells;
Busan;
DNA;
DNA Probes;
Escherichia coli*;
Escherichia*;
Humans;
Immune Sera;
Plasmids;
Serotyping*
- From:Journal of the Korean Society for Microbiology
1998;33(1):77-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Eighty-two strains of Escherichia coli isolated from urine specimens in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profiles, PFGE profiles, MRHA of human blood cells, HEp-2 cell adherence ability and reactivity to bfpA, LT, STh and STp DNA probes. The following results were obtained. Fifty-three of the eighty-two strains belonged to thirteen different 0 serotypes, twenty-nine strains could not be typed with the antisera used. Thirty strains (43.9%) were hemolysin producer. MRHA is present on twenty-nine strains (35.37%) of eighty-two strains. MRHA positive strains carry a plasmid of 60MDa, a putative factor involved in adherence. This plasmid might be specific for MRHA positive strains. MRHA positive strains were observed in serotype 01, 018, 055, 086a, 0119, 0126, and 0142. Twenty-six strains of E. coli showed three patterns of adherence to HEp-2 cells namely, localized, diffuse, and aggregative adhesion. Twenty-two strains hybridized with the bfpA probe, while all eighty-two strains did not hybridize with the probes, LT, STh, STp. The restriction fragment patterns of chromosomal DNA digested with AotI analysed by PFGE of hemolysin-producing E. coli ten strains were compared with eight different types. Three of E. coli serotype 01, 08 and 0126 showed the same chromosomal DNA fragment patterns.
