Histologic Analysis of Vocal Folds in Aging Rats
10.22469/jkslp.2020.31.2.66
- Author:
Sung-Chan SHIN
1
;
Ji Min KIM
;
Hyun-Keun KWON
;
Yong-Il CHEON
;
Byung-Joo LEE
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine, Busan, Korea
- Publication Type:Original Article
- From:Journal of the Korean Society of Laryngology Phoniatrics and Logopedics
2020;31(2):66-70
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Background and Objectives:Presbyphonia is characterized by hoarse, breathy, weak vocal intensity. Extracellular matrix (ECM) in lamina propria (LP) of the vocal folds play an important role in voice production, and change of ECM according to the aging leads to the presbyphonia. The aim of this study was to investigate the histologic analysis of aging vocal fold of rat.Materials and Method Six and twenty two months old Sprague-Dawley rats (n=8, each group) were used and classified into young (six months old rats) and old (twenty two months old rats) group. Histologic analysis and immunohistochemical staining for ECM of LP were performed.
Results:Overall cellular density was significantly decreased in old rat group. Elastin fibers of LP were significantly decreased in old rat group. Type I collagen was significantly increased in old rat group. Type III collagen did not show significant difference. Hyaluronic acids did not show significant difference in Alcian blue staining and immunohistochemical staining.
Conclusion:Decreased general cellular density and elastin fiber and increased type I collagen were observed in the LP of vocal folds of aging rats. These ECM changes might to contribute the aging voice.
