Expression of Platelet Derived Growth Factor-A, C and Platelet Derived Growth Factor Receptor-alpha in the Ischemia Reperfusion Renal Failure Model.
- Author:
Kyung Pyo KANG
1
;
Wom KIM
;
Chi Young MOON
;
Yong Bum JANG
;
Sik LEE
;
Sang Ok MOON
;
Mi Jeong SUNG
;
Duk Hoon KIM
;
Sung Kyew KANG
;
Sung Kwang PARK
Author Information
1. Department of Internal Medicine, Renal Regeneration Laboratory, Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonju, Korea. parksk@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Platelet-derived growth factor;
Ischemia/reperfusion injury;
Kidney failure;
Acute
- MeSH:
Acute Kidney Injury;
Blood Platelets*;
Blotting, Western;
Connective Tissue Cells;
Epithelial Cells;
Humans;
Immunohistochemistry;
Ischemia*;
Platelet-Derived Growth Factor*;
Proliferating Cell Nuclear Antigen;
Receptor Protein-Tyrosine Kinases;
Receptors, Platelet-Derived Growth Factor;
Regeneration;
Renal Insufficiency*;
Reperfusion Injury;
Reperfusion*;
Ribonucleases;
RNA, Messenger
- From:Korean Journal of Nephrology
2006;25(1):13-22
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGOUND: Platelet-derived growth factor (PDGF) is a widely expressed growth factor with both mitogenic and chemotactic activities in many connective tissue cell types. There are four members of PDGF family; PDGF-A, PDGF-B, PDGF-C, PDGF-D. Their biological effects are mediated via two tyrosine kinase receptors, PDGF receptor-alpha and PDGF receptor-beta, and PDGF-mediated signaling is critical for development of many organ systems and acquired disease. The aims of this study were to determine the changes of PDGF-A, PDGF-C and PDGF receptor (PDGFR)-alpha expression in ischemia reperfusion acute renal failure model. METHODS: We examined the expression and localization of PDGF-A, PDGF-C and PDGF receptor-alpha protein using Western blot analysis and immunohistochemistry and PDGF-C mRNA using RNase protection assay after ischemia reperfusion renal failure model. RESULTS: PDGF-A expression showed no change after ischemia reperfusion injury. Proliferating cell nuclear antigen expression increased at day 2 after ischemia reperfusion injury. PDGF-C expression increased at day 2 after ischemia reperfusion injury, and was localized in tubular epithelial cells of outer medulla. PDGFR-alpha increased at day 2 after ischemia reperfusion injury, and was localized in tubular interstitium of outer medulla. CONCLUSION: These results indicated that PDGF-C and PDGF receptor-alpha may have an important role in the renal regeneration after ischemia reperfusion renal injury.
