Comparison of Serologic Response of Hospitalized COVID-19 Patients Using 8 Immunoassays
- Author:
Sun Min LEE
1
;
In-Suk KIM
;
Seungjin LIM
;
Su Jin LEE
;
Won-Joo KIM
;
Kyung-Hwa SHIN
;
Soo Young MOON
;
Chulhun L. CHANG
Author Information
- Publication Type:Original Article
- From:Journal of Korean Medical Science 2021;36(9):e64-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:In Korea, there were issues regarding the use of immunoassays for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies to detect infection. So, we compared antibody results of eight kinds of commercial immunoassays using clinical remnant specimens.
Methods:We compared the results of several immunoassay kits tested on 40 serum samples from 15 confirmed patients and 86 remnant serum samples from clinical laboratory.Eight kinds of IVD kits—four enzyme-linked immunosorbent assay, two lateral flow rapid immunochromatographic assays, and two chemiluminescent immunoassays with one RUO kit were tested.
Results:Among 40 serum samples from 15 coronavirus disease 2019 (COVID-19) patients, 35 yielded at least one positive result for detecting antibodies in the combined assessment. There were inconsistent results in 12 (28%) samples by single immunoassay. Forty samples collected in 2019 before the first COVID-19 Korean case showed negative results except for one equivocal result.
Conclusion:The discrepant results obtained with different immunoassay kits in this study show that serological assessment of SARS-CoV-2 by a single immunoassay requires caution not only in detecting infection but also in assessing immunologic status.
