Effects of Dichloromethane Fraction of Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts.
10.5051/jkape.2003.33.2.259
- Author:
Young Joon LEE
1
;
Hee In CHOI
;
Yun Chul KIM
;
Hyung Keun YOU
;
Hyung Shik SHIN
Author Information
1. Department of Periodontology, School of Dentistry, Wonkwang University, Korea.
- Publication Type:Original Article
- Keywords:
Phlomidis Radix;
dichloromethane;
hFOB1;
mineralization
- MeSH:
Alkaline Phosphatase;
Fractures, Bone;
Hope;
Humans*;
Humidity;
Methylene Chloride*;
Osteoblasts*;
Osteogenesis*;
Osteoporosis;
Regeneration;
Wound Healing
- From:The Journal of the Korean Academy of Periodontology
2003;33(2):259-269
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The ideal goal of periodontal therapy is the regeneration of periodontal tissue and repair of function. Although it is very difficult to attain this goal, recent advances in periodontal wound healing concepts encourage hope reaching it. Recently many efforts are concentrated on the regeneration potential of material used in traditional Korean medicine. Phlomidis Radix has been used for the treatment of blood stasis, bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine effects of dichloromethane fraction Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts. Human fetal osteoblastic cell line(hFOB1 1.19 ; American Type Culture Collection, Manassas, VA) were used and cells were cultured containing DMEM and dichloromethane fraction Phlomidis Radix(100 ng/ml, 1 microgram/ml, 10 microgram/ml) at 34degrees C with 5% CO2 in 100% humidity. MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examnine the mineralization. Also bone calcification nodules were evaluated. The cellular activity of hFOB1 was increased in 100 ng/ml, 1 microgram/ml, 10 microgram/ml of dichloromethane fraction of Phlomidis Radix and especially significant increation was showed in 100 ng/ml of dichloromethane fraction of Phlomidis Radix at 6days (p<0.05). ALP level of hFOB1 was significantly increased in 100 ng/ml, 1 microgram/ml, 10 microgram/ml of dichloromethane fraction of Phlomidis Radix and especially more increation was showed in 10 microgram/ml of dichloromethane fraction of Phlomidis Radix (p<0.05). Calcification nodules of hFOB1 significantly increased in 10 microgram/ml of dichloromethane fraction of Phlomidis Radix at 21days of incubation (p<0.05). These results indicate that dichloromethane fraction of Phlomidis Radix has excellent effects on mineralization of hFOB1.
