Distinct Repopulation Activity in Hu-Mice between CBand LPB-CD34＋ Cells by Enrichment of Transcription Factors
- Author:
A-Reum HAN
1
;
Jeong Eun LEE
;
Min Ji LEE
;
Seung Young KO
;
Hyun Soo SHIN
;
Ji Yoon LEE
;
Dong Ryul LEE
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2021;14(2):203-211
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:Human CD34＋hematopoietic stem cells can reconstitute the human hematopoietic system when transplanted into immunocompromised mice after irradiation. Human leukapheresis peripheral blood (LPB)-and cord blood (CB)-derived CD34＋ cells have a similar capacity to reconstitute myeloid lineage cells in a humanized mice (hu-mice) model. However, potent stem cells, such as CB-CD34＋ cells, efficiently reconstitute the lymphoid system in vivo compared to LPB-CD34 ＋ cells. Modeling the human hematolymphoid system is vital for studying immune cell crosstalk in human xenografted mice, with CB-CD34＋ cells used as an optimized cell source because they are essential in reconstituting lymphoid lineage cells.
Methods:and Results: In this study, we established hu-mice that combined human characteristics with long-term survival and investigated the efficiency of the engraftment of lymphoid lineage cells derived from LPB- and CB-CD34＋cells in the bone marrow, spleen, and LPB. We found an overall increase in the transcriptional activity of lymphoid lineage genes in CB-CD34＋ cells. Our results revealed that potent CB-CD34＋ cells displaying a general upregulation of the expression of genes involved in lymphopoiesis could contribute to the hematolymphoid system in the humanized mice model with longevity.
Conclusions:Our data suggest that humanized mouse model by usage of CB-CD34 ＋ cells displaying high expression of TFs for lymphoid lineage cells can contribute to study the immune response against lymphocytes.
