Umbilical Cord Mesenchymal Stem Cells for Inhibiting the Fibrosis and Autoimmune Development in HOCl-Induced Systemic Scleroderma Mouse Model
- Author:
Xin JIN
1
;
Jiali HOU
;
Ke ZHENG
;
Dan WEI
;
Ali ZHANG
;
Siqi WANG
;
Hua MEI
;
Chuang LI
;
Lamei CHENG
;
Xuan SUN
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2021;14(3):262-274
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:Systemic scleroderma (SSc) is a rare and serious connective tissue disease, an autoimmune disease, and a rare refractory disease. In this study, preventive effect of single systemic human umbilical cord mesenchymal stem cells (UC-MSCs) transfusion on SSc was preliminarily explored.
Methods:and Results: SSc mouse model was established by daily intradermal injection of Hypochlorite (HOCl). SSc mice were treated by single transfusion of UC-MSCs at 0.625×10 5 , 2.5×105 and 1×106 respectively. At the 42nd day of intradermal injection of HOCl, the symptoms showed up by skin and alveolar wall thickening, lymphocytic infiltration, increased collagen in skin/lung, and the increased proportion of CD3 ＋ CD4＋ CD25＋ FoxP3＋ cells (a Treg subset) in spleen. After UC-MSCs transfusion, the degree of skin thickening, alveolar wall thickening and lymphocyte infiltration were decreased, the collagen sedimentation in skin/lung was decreased, and the proportion of CD3＋ CD4＋ CD25＋FoxP3＋ cells was decreased.
Conclusions:UC-MSC can achieve a preventive effect in SSc mice by fibrosis attenuation and immunoregulation.
