Anticancer effects of D-pinitol in human oral squamous carcinoma cells
10.11620/IJOB.2020.45.4.152
- Author:
Hyun-Chul SHIN
1
;
Tea-Hyun BANG
;
Hae-Mi KANG
;
Bong-Soo PARK
;
In-Ryoung KIM
Author Information
1. Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea
- Publication Type:Original Article
- From:International Journal of Oral Biology
2020;45(4):152-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
D-pinitol is an analog of 3-methoxy-D-chiro-inositol found in beans and plants. D-pinitol has anti-inflammatory, antidiabetic, and anticancer effects. Additionally, D-pinitol induces apoptosis and inhibits metastasis in breast and prostate cancers. However, to date, no study has investigated the anticancer effects of D-pinitol in oral cancer. Therefore, in this study, whether the anticancer effects of D-pinitol induce apoptosis, inhibit the epithelialto-mesenchymal transition (EMT), and arrest cell cycle was investigated in squamous epithelial cells. D-pinitol decreased the survival and cell proliferation rates of CAL-27 and Ca9-22 oral squamous carcinoma cells in a concentration- and time-dependent manner. Evidence of apoptosis, including nuclear condensation, poly (ADP-ribose) polymerase, and caspase-3 fragmentation, was also observed. D-pinitol inhibited the migration and invasion of both cell lines. In terms of EMT-related proteins, E-cadherin was increased, whereas N-cadherin, Snail, and Slug weredecreased. D-pinitol also decreased the expression of cyclin D1, a protein involved in the cell cycle, but increased the expression of p21, a cyclin-dependent kinase inhibitor. Hence, D-pinitol induces apoptosis and cell cycle arrest in CAL-27 and Ca9-22 cells, demonstrating an anticancer effect by decreasing the EMT.
