Alteration of cellular events in tooth development by chemical chaperon, Tauroursodeoxycholic acid treatment
10.11620/IJOB.2020.45.4.190
- Author:
Eui-Seon LEE
1
;
Yam Prasad ARYAL
;
Tae-Young KIM
;
Elina POKHAREL
;
Harim KIM
;
Shijin SUNG
;
Wern-Joo SOHN
;
Youngkyun LEE
;
Chang-Hyeon AN
;
Jae-Young KIM
Author Information
1. Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea
- Publication Type:Original Article
- From:International Journal of Oral Biology
2020;45(4):190-196
- CountryRepublic of Korea
- Language:English
-
Abstract:
Several factors, including genetic and environmental insults, impede protein folding and secretion in the endoplasmic reticulum (ER). Accumulation of unfolded or mis-folded protein in the ER manifests as ER stress. To cope with this morbid condition of the ER, recent data has suggested that the intracellular event of an unfolded protein response plays a critical role in managing the secretory load and maintaining proteostasis in the ER. Tauroursodeoxycholic acid (TUDCA) is a chemical chaperone and hydrophilic bile acid that is known to inhibit apoptosis by attenuating ER stress. Numerous studies have revealed that TUDCA affects hepatic diseases, obesity, and inflammatory illnesses. Recently, molecular regulation of ER stress in tooth development, especially during the secretory stage, has been studied. Therefore, in this study, we examined the developmental role of ER stress regulation in tooth morphogenesis using in vitro organ cultivation methods with a chemical chaperone treatment, TUDCA. Altered cellular events including proliferation, apoptosis, and dentinogenesis were examined using immunostaining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In addition, altered localization patterns of the formation of hard tissue matrices related to molecules, including amelogenin and nestin, were examined to assess their morphological changes. Based on our findings, modulating the role of the chemical chaperone TUDCA in tooth morphogenesis, especially through the modulation of cellular proliferation and apoptosis, could be applied as a supporting data for tooth regeneration for future studies.
