Potential Role of Cytosolic RNA Sensor MDA5 as an Inhibitor for Keratinocyte Differentiation in the Pathogenesis of Psoriasis
- Author:
Dong-Kyun HONG
1
;
Mi-Ra CHOI
;
Yul-Lye HWANG
;
Jae Kyung LEE
;
Young LEE
;
Young-Joon SEO
;
Sooil KIM
;
Young-Ho LEE
;
Chang-Deok KIM
;
Jeung-Hoon LEE
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:Annals of Dermatology 2021;33(4):339-344
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Psoriasis is a chronic inflammatory skin disease. The etiology of psoriasis is not fully understood, but the genetic background is considered to be the most important factor. To date, many psoriasis-related genes have been discovered, but the role of many important genes has not been well understood.
Objective:The purpose of this study is to uncover possible roles of MDA5 in psoriasis.
Methods:Expression of MDA5 was investigated using immunohistochemistry. Then, MDA5 was overexpressed in keratinocytes using a recombinant adenovirus.
Results:As a result of immunohistochemical staining, the expression of MDA5 was significantly increased in the epidermis of psoriasis compared to normal skin. Similarly, the expression of MDA5 was increased in imiquimod-induced psoriasiform dermatitis model. In cultured keratinocytes, toll-like receptor 3 agonist poly(I:C) induced expression of MDA5 at both mRNA and protein levels. When MDA5 was overexpressed using a recombinant adenovirus, poly(I:C)-induced cytokine expression was significantly increased. Finally, MDA5 overexpression significantly inhibited calcium-induced differentiation of keratinocytes.
Conclusion:These results suggest that MDA5 increases in psoriasis and negatively regulates keratinocyte differentiation.
