Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation.
10.1016/j.apsb.2021.03.011
- Author:
Qi LV
1
;
Yao XING
1
;
Jian LIU
1
;
Dong DONG
1
;
Yue LIU
1
;
Hongzhi QIAO
1
;
Yinan ZHANG
1
;
Lihong HU
1
Author Information
1. Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, Stake Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
3-MC, 3-methylcholanthrene;
5-ASA, 5-aminosalicylic acid;
AIM2, absent in melanoma 2;
ATG5, autophagy-related protein 5;
ATG7, autophagy-related protein 7;
ATP, adenosine triphosphate;
Autophagy;
BMDMs, bone marrow-derived macrophages;
CETSA, cellular thermal shift assay;
CHX, cycloheximide;
ChIP, chromatin immunoprecipitation;
Colitis;
DAI, disease activity index;
DAMPs, damage-associated molecular patterns;
DMSO, dimethyl sulfoxide;
DSS, dextran sulfate sodium;
DTT, dithiothreitol;
ECL, enhanced chemiluminescent;
EDTA, ethylenediaminetetraacetic acid;
ELISA, enzyme-linked immunosorbent assay;
EZH2;
EZH2, enhancer of zeste homolog 2;
FBS, fetal bovine serum;
H&E, hematoxylin and eosin;
LPS, lipopolysaccharide;
Lonicerin;
M-CSF, macrophage colony stimulating factor;
MDP, muramyldipeptide;
MPO, myeloperoxidase;
MSU, monosodium urate crystals;
NLRP3 inflammasome;
NLRP3, nucleotide-binding domain-like receptors family pyrin domain containing 3;
PAMPs, pathogen-associated molecular patterns;
PMA, phorbol myristate acetate;
PMSF, phenylmethanesulfonyl fluoride;
PRC2, polycomb repressive complex 2;
RMSD, root mean-square deviation;
RMSF, root mean-square fluctuation;
SIP, solvent-induced protein precipitation;
TEM, transmission electron microscopy;
UC, ulcerative colitis
- From:
Acta Pharmaceutica Sinica B
2021;11(9):2880-2899
- CountryChina
- Language:English
-
Abstract:
Aberrant activation of NLRP3 inflammasome in colonic macrophages strongly associates with the occurrence and progression of ulcerative colitis. Although targeting NLRP3 inflammasome has been considered to be a potential therapy, the underlying mechanism through which pathway the intestinal inflammation is modulated remains controversial. By focusing on the flavonoid lonicerin, one of the most abundant constituents existed in a long historical anti-inflammatory and anti-infectious herb
