Enzyme-instructed hybrid nanogel/nanofiber oligopeptide hydrogel for localized protein delivery.
10.1016/j.apsb.2020.11.010
- Author:
Tianyue JIANG
1
;
Yudi MA
1
;
Xiao XU
2
;
Qingchun JI
1
;
Mingxing FENG
1
;
Cheng CHENG
1
;
Yang FENG
2
;
Bingfang HE
1
;
Ran MO
2
Author Information
1. School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China.
2. State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Center of Advanced Pharmaceuticals and Biomaterials, China Pharmaceutical University, Nanjing 210009, China.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cancer therapy;
Cytochrome c;
Enzymatic catalysis;
Local administration;
Nanogel;
Oligopeptide hydrogel;
Protein delivery
- From:
Acta Pharmaceutica Sinica B
2021;11(7):2070-2079
- CountryChina
- Language:English
-
Abstract:
Enzyme-catalysis self-assembled oligopeptide hydrogel holds great interest in drug delivery, which has merits of biocompatibility, biodegradability and mild gelation conditions. However, its application for protein delivery is greatly limited by inevitable degradation of enzyme on the encapsulated proteins leading to loss of protein activity. Moreover, for the intracellularly acted proteins, cell membrane as a primary barrier hinders the transmembrane delivery of proteins. The internalized proteins also suffer from acidic and enzymatic degradation in endosomes and lysosomes. We herein develop a protease-manipulated hybrid nanogel/nanofiber hydrogel for localized delivery of intracellularly acted proteins. The embedded polymeric nanogels (CytoC/aNGs) preserve activity of cytochrome
