Inhibition of LIM kinase reduces contraction and proliferation in bladder smooth muscle.
- Author:
Qingfeng YU
1
;
Chengjie WU
1
;
Yeda CHEN
1
;
Bingsheng LI
2
;
Ruixiao WANG
2
;
Ru HUANG
2
;
Xuechun LI
1
;
Di GU
1
;
Xiaolong WANG
2
;
Xiaolu DUAN
1
;
Shujue LI
1
;
Yang LIU
1
;
Wenqi WU
1
;
Martin HENNENBERG
2
;
Guohua ZENG
1
Author Information
- Publication Type:Journal Article
- Keywords: 4E-BP1, 4E-binding protein 1; ADF, actin depolymerizing factors; BOO, bladder outlet obstruction; BPH, benign prostatic hyperplasia; Bladder smooth muscle contraction; CCK-8, Cell Counting Kit-8; Cofilin phosphorylation; Ct, number of cycles; DMSO, dimethyl sulfoxide; EdU, 5-ethynyl-2′-deoxyuridine; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; H&E, hematoxylin and eosin; HBSMCs, human bladder smooth muscle cells; HRP, horseradish peroxidase; LIMK; LIMKs, LIM kinases; LUTS, lower urinary tract symptoms; Lower urinary tract symptoms (LUTS); MLC, myosin light chain; MW, molecular weight; MYPT1, myosin-binding subunit; OAB, overactive bladder; Overactive bladder (OAB); PCNA, proliferating cell nuclear antigen; RT-qPCR, reverse transcription and quantitative polymerase chain reaction; STK16, serine/threonine kinase 16; TESK1, testicular protein kinase 1; TXA2, thromboxane A2; WST-8, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt; siRNA, small interfering RNA
- From: Acta Pharmaceutica Sinica B 2021;11(7):1914-1930
- CountryChina
- Language:English
- Abstract: Overactive bladder (OAB) is the most bothersome symptom in lower urinary tract symptoms (LUTS). Current pharmacologic treatment aims to inhibit detrusor contraction; however, shows unsatisfied efficacy and high discontinuation rate. LIM kinases (LIMKs) promote smooth muscle contraction in the prostate; however, their function in the bladder smooth muscle remains unclear. Here, we studied effects of the LIMK inhibitors on bladder smooth muscle contraction and proliferation both
