A combined quality evaluation method that integrates chemical constituents, appearance traits and origins of raw Rehmanniae Radix pieces.
10.1016/S1875-5364(21)60056-0
- Author:
Min GU
1
;
Yi-Ping YUAN
2
;
Zi-Nan QIN
2
;
Yan XU
2
;
Nan-Nan SHI
3
;
Yan-Ping WANG
3
;
Hua-Qiang ZHAI
4
;
Zhong-Zhi QIAN
5
Author Information
1. Standardization Research Center of Traditional Chinese Medicine Dispensing, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: 2781352102@qq.com.
2. Standardization Research Center of Traditional Chinese Medicine Dispensing, Beijing University of Chinese Medicine, Beijing 102488, China.
3. Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
4. Standardization Research Center of Traditional Chinese Medicine Dispensing, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: jz711@qq.com.
5. National Pharmacopoeia Commission, Beijing 100061, China. Electronic address: n201801@126.com.
- Publication Type:Journal Article
- Keywords:
Appearance traits;
Bioactive constituents;
Different origins;
Quality evaluation;
Raw Rehmanniae Radix
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/analysis*;
Medicine, Chinese Traditional;
Plant Roots/chemistry*;
Principal Component Analysis;
Quality Control;
Rehmannia/chemistry*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(7):551-560
- CountryChina
- Language:English
-
Abstract:
The quality control of Chinese herbal medicine is a current challenge for the internationalization of traditional Chinese medicine. Traditional quality evaluation methods lack quantitative analysis, while modern quality evaluation methods ignore the origins and appearance traits. Therefore, an integrated quality evaluation method is urgent in need. Raw Rehmanniae Radix (RRR) is commonly used in Chinese herbal medicine. At present, much attention has been drwan towards its quality control, which however is limited by the existing quality evaluation methods. The present study was designed to establish a comprehensive and practical method for the quality evaluation and control of RRR pieces based on its chemical constituents, appearance traits and origins. Thirty-three batches of RRR pieces were collected from six provinces, while high-performance liquid chromatography (HPLC) was applied to determine the following five constituents, including catalpol, rehmannioside A, rehmannioside D, leonuride and verbascoside in RRR pieces. Their appearance traits were quantitatively observed. Furthermore, correlation analysis, principal components analysis (PCA), cluster analysis and t-test were performed to evaluate the qualities of RRR pieces. These batches of RRR pieces were divided into three categories: samples from Henan province, samples from Shandong and Shanxi provinces, and those from other provinces. Furthermore, the chemical constituents and appearance traits of RRR pieces were significantly different from diverse origins. The combined method of chemical contituents, appearance traits and origins can distinguish RRR pieces with different qualities, which provides basic reference for the quality control of Chinese herbal medicine.
