Three new polyketides from vasR2 gene over-expressed mutant strain of Verrucosispora sp. NS0172.
10.1016/S1875-5364(21)60053-5
- Author:
Li-Ping DAI
1
;
Wen LI
2
;
Hao-Xin WANG
3
;
Chun-Hua LU
4
Author Information
1. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
3. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. Electronic address: wanghaoxin@sdu.edu.cn.
4. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China. Electronic address: ahua0966@sdu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Ansamycins;
SARP family regulatory gene;
Verrucosispora sp. NS0172
- MeSH:
Micromonosporaceae/metabolism*;
Multigene Family;
Polyketides/metabolism*;
Rifabutin/metabolism*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(7):536-539
- CountryChina
- Language:English
-
Abstract:
Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster. In the curret study, the SARP family regulatory gene, vasR2, was over-expressed in strain Verrucosispora sp. NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated. Two tetraketides (1 and 2) and a triketide (3) ansamycins, together with five known compounds (4-8), were isolated and elucidated from strain NS0172OEvasR2. Their NMR data were completely assigned by analysis of their HR-ESI-MS and
