A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice.
- Author:
Victor Abou NEHMI
1
;
Gilson Masahiro MURATA
2
;
Ruan Carlos Macêdo de MORAES
1
;
Gabriely Cristina Alves LIMA
1
;
Danielle Araujo DE MIRANDA
3
;
Katrin RADLOFF
4
;
Raquel Galvão Figuerêdo COSTA
5
;
Joyce de Cassia Rosa de JESUS
1
;
Jéssica Alves DE FREITAS
2
;
Nayara Izabel VIANA
6
;
Ruan PIMENTA
7
,
8
;
Katia Ramos Moreira LEITE
6
;
José Pinhata OTOCH
9
,
10
;
Ana Flávia Marçal PESSOA
9
,
11
,
12
Author Information
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords: Inflammation; Mice; Mitochondrial biogenesis; Obese; Prebiotic; Silymarin; Steatosis; Supplement
- MeSH: Animals; Antioxidants; Dietary Supplements; Glucans; Hypothalamo-Hypophyseal System; Liver; Mice; Mice, Inbred C57BL; Mice, Obese; Milk Thistle; Minerals; Pituitary-Adrenal System; Prebiotics; Saccharomyces cerevisiae
- From: Journal of Integrative Medicine 2021;19(5):439-450
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To evaluate the synergic effects of a novel oral supplement formulation, containing prebiotics, yeast β-glucans, minerals and silymarin (Silybum marianum), on lipid and glycidic metabolism, inflammatory and mitochondrial proteins of the liver, in control and high-fat diet-induced obese mice.
METHODS:After an acclimation period, 32 male C57BL/6 mice were divided into the following groups: nonfat diet (NFD) vehicle, NFD supplemented, high-fat diet (HFD) vehicle and HFD supplemented. The vehicle and experimental formulation were administered orally by gavage once a day during the last four weeks of the diet (28 consecutive days). We then evaluated energy homeostasis, inflammation, and mitochondrial protein expression in these groups of mice.
RESULTS:After four weeks of supplementation, study groups experienced reduced glycemia, dyslipidemia, fat, and hepatic fibrosis levels. Additionally, proliferator-activated receptor-α, AMP-activated protein kinase-1α, peroxisome proliferator-activated receptor γ co-activator-1α, and mitochondrial transcription factor A expression levels were augmented; however, levels of inhibitor of nuclear factor-κB kinase subunit α and p65 nuclear factor-κB expression, and oxidative markers were reduced. Notably, the cortisol/C-reactive protein ratio, a well-characterized marker of the hypothalamic-pituitary-adrenal axis immune interface status, was found to be modulated by the supplement.
CONCLUSION:We discovered that the novel supplement was able to modify different antioxidant, metabolic and inflammatory pathways, improving the energy homeostasis and inflammatory status, and consequently alleviated hepatic steatosis.