Resveratrol promotes the survival and neuronal differentiation of hypoxia-conditioned neuronal progenitor cells in rats with cerebral ischemia.
10.1007/s11684-021-0832-y
- Author:
Yao YAO
1
;
Rui ZHOU
1
;
Rui BAI
2
;
Jing WANG
1
;
Mengjiao TU
1
;
Jingjing SHI
1
;
Xiao HE
1
;
Jinyun ZHOU
1
;
Liu FENG
1
;
Yuanxue GAO
1
;
Fahuan SONG
1
;
Feng LAN
2
;
Xingguo LIU
3
;
Mei TIAN
4
;
Hong ZHANG
5
Author Information
1. Department of Nuclear Medicine and PET-CT Center, The Second Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
2. Beijing Laboratory for Cardiovascular Precision Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
3. Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
4. Department of Nuclear Medicine and PET-CT Center, The Second Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China. meitian@zju.edu.cn.
5. Department of Nuclear Medicine and PET-CT Center, The Second Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China. hzhang21@zju.edu.cn.
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
mitochondrial metabolism;
neuronal differentiation;
neuronal progenitor cells;
positron emission tomography;
resveratrol
- MeSH:
Animals;
Brain Ischemia/drug therapy*;
Cell Differentiation;
Hypoxia;
Neurons;
Rats;
Resveratrol/pharmacology*
- From:
Frontiers of Medicine
2021;15(3):472-485
- CountryChina
- Language:English
-
Abstract:
Hypoxia conditioning could increase the survival of transplanted neuronal progenitor cells (NPCs) in rats with cerebral ischemia but could also hinder neuronal differentiation partly by suppressing mitochondrial metabolism. In this work, the mitochondrial metabolism of hypoxia-conditioned NPCs (hcNPCs) was upregulated via the additional administration of resveratrol, an herbal compound, to resolve the limitation of hypoxia conditioning on neuronal differentiation. Resveratrol was first applied during the in vitro neuronal differentiation of hcNPCs and concurrently promoted the differentiation, synaptogenesis, and functional development of neurons derived from hcNPCs and restored the mitochondrial metabolism. Furthermore, this herbal compound was used as an adjuvant during hcNPC transplantation in a photothrombotic stroke rat model. Resveratrol promoted neuronal differentiation and increased the long-term survival of transplanted hcNPCs. 18-fluorine fluorodeoxyglucose positron emission tomography and rotarod test showed that resveratrol and hcNPC transplantation synergistically improved the neurological and metabolic recovery of stroke rats. In conclusion, resveratrol promoted the neuronal differentiation and therapeutic efficiency of hcNPCs in stroke rats via restoring mitochondrial metabolism. This work suggested a novel approach to promote the clinical translation of NPC transplantation therapy.
