Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws.
10.1038/s41368-021-00127-3
- Author:
Ming MA
1
;
Lu LIU
2
;
Ruirui SHI
1
;
Jianyun ZHANG
1
;
Xiaotian LI
1
;
Xuefen LI
3
;
Jiaying BAI
1
;
Jianbin WANG
4
;
Yanyi HUANG
5
;
Tiejun LI
6
Author Information
1. Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, China.
2. Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing, China.
3. Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, China.
4. School of Life Sciences and Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Beijing, China. jianbinwang@tsinghua.edu.cn.
5. Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing, China. yanyi@pku.edu.cn.
6. Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, China. litiejun22@vip.sina.com.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA Copy Number Variations;
Diagnosis, Differential;
Fibroma, Ossifying/genetics*;
Fibrous Dysplasia of Bone/genetics*;
Galactosyltransferases;
Humans;
Jaw;
Neoplasm Recurrence, Local;
Nuclear Proteins
- From:
International Journal of Oral Science
2021;13(1):21-21
- CountryChina
- Language:English
-
Abstract:
Ossifying fibroma (OF) and fibrous dysplasia (FD) are two fibro-osseous lesions with overlapping clinicopathological features, making diagnosis challenging. In this study, we applied a whole-genome shallow sequencing approach to facilitate differential diagnosis via precise profiling of copy number alterations (CNAs) using minute amounts of DNA extracted from morphologically correlated microdissected tissue samples. Freshly frozen tissue specimens from OF (n = 29) and FD (n = 28) patients were obtained for analysis. Lesion fibrous tissues and surrounding normal tissues were obtained by laser capture microdissection (LCM), with ~30-50 cells (5 000-10 000 µm
