Inhibitory mechanism of angiotensin-converting enzyme inhibitory peptides from black tea.

Author: Yating LU ¹ ; Yu WANG ¹ ; Danyi HUANG ¹ ; Zhuang BIAN ¹ ; Peng LU ² ; Dongmei FAN ¹ ; Xiaochang WANG ¹
Author Information

1. Tea Research Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
2. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Publication Type:Journal Article
Keywords: Angiotensin-1-converting enzyme (ACE) inhibitory peptide; Black tea; Kinetic study; Molecular docking; Molecular dynamic (MD) simulation
From: Journal of Zhejiang University. Science. B 2021;22(7):575-589
CountryChina
Language:English
Abstract: The aim of this work is to discover the inhibitory mechanism of tea peptides and to analyse the affinities between the peptides and the angiotensin-converting enzyme (ACE) as well as the stability of the complexes using in vitro and in silico methods. Four peptide sequences identified from tea, namely peptides I, II, III, and IV, were used to examine ACE inhibition and kinetics. The half maximal inhibitory concentration (IC