Effect of Astaxanthin on Antioxidant Enzyme Activities in Suspended Leukocyte-Depleted Red Blood Cells Stored for Transfusion.
10.19746/j.cnki.issn.1009-2137.2021.04.047
- Author:
Su-Ling WANG
1
;
Qie WANG
2
Author Information
1. Matching Laboratory, Hebei Blood Center, Shijiazhuang 050071, Hebei Province, China.
2. Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China,E-mail: wangqie1012@163.com.
- Publication Type:Journal Article
- MeSH:
Antioxidants;
Catalase/metabolism*;
Erythrocytes;
Leukocytes;
Oxidative Stress;
Superoxide Dismutase/metabolism*;
Xanthophylls
- From:
Journal of Experimental Hematology
2021;29(4):1312-1317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of astaxanthin (ASTA) on the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in suspended leukocyte-depleted red blood cells stored for transfusion.
METHODS:The suspended leukocyte-depleted red blood cells were randomly divided into group A, B, C and D. The ASTA was added into preservation solution of suspended leukocyte-depleted red blood cells of group B, C and D with the final concentration 5, 10 and 20 μmol/L, respectively, while DMSO was added into cells of group A in the same volume. After 7, 14, 28 and 42 days of storage, the reactive oxygen species (ROS) content in red blood cells was detected by fluorescence microplate reader, malondialdehyde (MDA) content was detected by thiobarbituric acid (TBA) method, activity of SOD was detected by xanthine oxidase method, the activity of CAT was detected by visible light method, and activity of GSH-Px was detected by colorimetry.
RESULTS:After 7, 14, 28 and 42 days of storage, the contents of ROS and MDA in suspended red blood cells of group B, C and D were significantly lower(P<0.05), while the activities of SOD and GSH-Px were higher than those of group A(P<0.05); and CAT activity in cells treated by ASTA was significantly higher at 28 and 42 days of storage in comparison with that of group A(P<0.05). There were positive correlations between the ROS, MDA content in suspended red blood cells of group A, B, C, D and storage time(P<0.01), while negative correlation between SOD, CAT, GSH-Px activity and storage time(P<0.01).
CONCLUSION:ASTA can decrease the oxidative stress level and peroxide damage degree by increasing the antioxidant enzyme activities in suspended leukocyte-depleted red blood cells during storage.
