Distribution of ETV6-ABL Fusion Gene in Hematopoietic Cell Populations of Myeloproliferative Neoplasm.
10.19746/j.cnki.issn.1009-2137.2021.04.034
- Author:
Yuan CHEN
1
;
Qian LIU
1
;
Yan LI
2
;
Hui WEI
3
;
Qing RAO
1
;
Min WANG
1
;
Jian-Xiang WANG
4
Author Information
1. State Key Laboratory of Experimental Hematology, Tianjin 300020, China.
2. National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
3. State Key Laboratory of Experimental Hematology, Tianjin 300020, China,National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China,E-mail: weihui@ihcams.ac.cn.
4. State Key Laboratory of Experimental Hematology, Tianjin 300020, China,National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Fusion Proteins, bcr-abl/genetics*;
Genes, abl;
Hematopoietic Stem Cell Transplantation;
Humans;
Imatinib Mesylate;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics*;
Male;
Myeloproliferative Disorders/genetics*
- From:
Journal of Experimental Hematology
2021;29(4):1242-1246
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression level of ETV6-ABL fusion gene in different cell populations in patients with myeloproliferative neoplasm (MPN) and therapeutic effect of tyrosine kinase inhibitor (TKI).
METHODS:A 42-year-old man who presented with fever, marked leukocytosis and chronic myelogenous leukemia (CML) like MPN was reported. ETV6-ABL fusion gene was detected by real-time PCR and confirmed by direct sequencing. ETV6-ABL mRNA expression in each cell population sorted from peripheral blood by flow cytometry was detected by real-time PCR.
RESULTS:ETV6-ABL fusion gene was found out in bone marrow cells and confirmed as type A by direct sequencing. ETV6-ABL fusion gene transcript level in polymorphonuclear cells was nearly 3.6-fold relative to that in total cells, which was significantly higher than that in T cell, B cell and monocyte subsets. The complete blood count (CBC) returned to normal level after treatment with imatinib (400 mg) daily for three months. After TKI treatment for 6 months, the ratio of ETV6-ABL/ABL decreased from 174.1% to 1.9%.
CONCLUSION:ETV6-ABL fusion gene positive MPN may have a CML clinical presentation and is sensitive to TKI. ETV6-ABL fusion gene is specifically expressed in polymorphonuclear cells.
