Analysis of Proliferation Characters of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Myelodysplastic Syndrome.
10.19746/j.cnki.issn.1009-2137.2021.04.031
- Author:
Yan-Bin PANG
1
;
Min-Jie ZHANG
2
;
Su-Rong LI
3
;
Jin ZHANG
1
;
Xue-Lian ZHAO
1
;
Jiang-Bo ZHANG
1
;
Ji-Yuan WANG
2
;
Guo-Tao FANG
4
;
Rui ZHANG
5
;
Li-Xia FAN
6
Author Information
1. Department of Hematology, The Afilliatied Hospital of Hebei Universtity, Baoding 071000, Hebei Province, China.
2. Department of Obstetrics, Baoding Maternal and Child Health Hospital, Baoding 071000, Hebei Province, China.
3. Department of Internal Medicine, The People's Hospital of Luancheng District, Shijiazhuang 051430, Hebei Province,China.
4. Department of Medical Oncology, The Afilliatied Hospital of Hebei Universtity, Baoding 071000, Hebei Province, China.
5. Department of General Surgery, The Afilliatied Hospital of Hebei Universtity, Baoding 071000, Hebei Province, China.
6. Department of Hematology, The Afilliatied Hospital of Hebei Universtity, Baoding 071000, Hebei Province, China,E-mail:13582097209@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Bone Marrow Cells;
Cell Proliferation;
Humans;
Mesenchymal Stem Cells;
Myelodysplastic Syndromes
- From:
Journal of Experimental Hematology
2021;29(4):1224-1230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the proliferation potential of bone marrow-derived mesenchymal stem cells (MSC) in patients with myelodysplastic syndrome (MDS).
METHODS:The MSC derived from the 24 patients with newly diagnosed MDS (MDS-MSC group) and MSC derived from 15 patients with nutritional anemia (control group) in the Affiliated Hospital of Hebei University were used as the research objects. The proliferation potential of MSC was analyzed by colony-forming unit assay, doubling time, cumulative passaging, cell number after 10 days of culture with equal amount of MSC and MTT experiment. The mechanism of abnormal proliferation was analyzed by cell cycle experiment, apoptosis experiment and p21 gene expression assay.
RESULTS:In the colony forming unit assay, the number of MDS-MSC colonies was 4.44±2.51, which was significantly lower than that of the control group (12.44±2.55)(P<0.01); the doubling time of MDS-MSC group was significantly longer than that of the control group (7.80±3.26 vs 3.63±0.85) (P<0.01); the number of MDS-MSC in 5×10
CONCLUSION:The proliferative capability of MDS-MSC is significantly reduced, which relates with the arrest of cell cycle in G
