Effect of Down-Regulation of LncRNA-HOTAIRM1 to Proliferation, Apoptosis and KIT/AKT Pathway of Jurkat Cells.
10.19746/j.cnki.issn.1009-2137.2021.04.016
- Author:
Yu-Ru LI
1
;
Wen-Jing YAN
1
;
Li-Li CAI
1
;
Xin-Li DENG
2
Author Information
1. Department of Laboratory Medicine, Second Medical Center of PLA General Hospital, Beijing 100853, China.
2. Department of Laboratory Medicine, Second Medical Center of PLA General Hospital, Beijing 100853, China E-mail:ruyuli@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Down-Regulation;
Humans;
Jurkat Cells;
Proto-Oncogene Proteins c-akt/metabolism*;
RNA, Long Noncoding/genetics*
- From:
Journal of Experimental Hematology
2021;29(4):1123-1128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effects of down-regulation of long non-coding RNA HOX antisense intergenic RNA myeloid 1 (LncRNA-HOTAIRM1) to the proliferation and apoptosis of Jurkat in human leukemia T lymphocytes, and explore its mechanism.
METHODS:Jurkat cells were cultured in vitro and randomly divided into control group, HOTAIRM1 siRNA-NC group and HOTAIRM1 siRNA group; the expressions of LncRNA-HOTAIRM1 mRNA, KIT receptor tyrosine kinase (KIT) mRNA and serine threonine kinase (AKT) mRNA in Jurkat cells were detected by real-time fluorescence quantification (RT-qPCR); the proliferation of Jurkat cells in each groups was detected by CCK-8 method; the apoptosis of Jurkat cells in each groups was detected by Annexin V-FITC/PI double staining; the expressions of KIT, AKT, p-KIT, p-AKT, B-lymphoma-2 gene (BCL-2) and Caspase-3 were detected by Western blot.
RESULTS:Compared with the cells in the control group and HOTAIRM1 siRNA-NC group, the expression level of LncRNA-HOTAIRM1 mRNA, cell survival rate, expression levels of KIT mRNA, AKT mRNA, p-KIT, p-AKT and BCL-2 proteins in Jurkat cells in HOTAIRM1 siRNA group were significantly lower (P<0.05), while the expression level of Cleared Caspase-3 protein and Jurkat cell apoptosis rate were significantly higher (P<0.05).
CONCLUSION:LncRNA-HOTAIRM1 may inhibit Jurkat cell proliferation and induce apoptosis through KIT/AKT signaling pathway.
