Effect and Mechanism of Sulforaphane on G
10.19746/j.cnki.issn.1009-2137.2021.04.006
- Author:
Fan-Ping WANG
1
,
2
;
Cai-Juan QIAO
1
,
3
;
Yan-Wei SUN
1
,
3
;
Xiang-Yang LI
1
,
3
;
Xiao-Yu HUANG
1
,
3
;
Wen-Rui ZHANG
1
,
3
;
Xia WANG
1
,
3
;
Ming-Yong WANG
1
,
4
Author Information
1. School of Laboratory Medicine, Xinxiang Medical University
2. Xinxiang 453003, Henan Province, China,Henan Key Laboratory of Immunology and Targeted Drug, Xinxiang 453003, Henan Province, China,Xinxiang Key Laboratory of Immunoregulation and Molecular Diagnostics, Xinxiang 453003, Henan Province, China.
3. Xinxiang 453003, Henan Province, China.
4. Xinxiang 453003, Henan Province, China,Henan Key Laboratory of Immunology and Targeted Drug, Xinxiang 453003, Henan Province, China,Xinxiang Key Laboratory of Immunoregulation and Molecular Diagnostics, Xinxiang 453003, Henan Province, China,E-mail：wmy118@126.com.
- Publication Type:Journal Article
- MeSH:
Cell Cycle;
Humans;
Isothiocyanates/pharmacology*;
Leukemia, Myeloid, Acute;
Mitosis;
Sulfoxides
- From:
Journal of Experimental Hematology
2021;29(4):1050-1055
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of sulforaphane (SFN) on G
METHODS:KG1a and KG1cells were treated by different concentrations of SFN for 48 h. Flow cytometry (FCM) was used to analyze the phase distribution of cell cycle. High-throughput sequencing was used to detect the effect of SFN on the expression of cell cycle related genes in KG1a cells. The mRNA expression of P53, P21, CDC2 and CyclinB1 were detected by qPCR. The protein expression of P53, CDC2, P-CDC2 and CyclinB1 were detected by Western blot.
RESULTS:Cells in the G
CONCLUSION:SFN induces leukemia cells to block in G
