Alternative Splicing Analysis of LACTB Gene and Expression Characteristics of Different Transcripts in Leukemia Cell Lines.
10.19746/j.cnki.issn.1009-2137.2021.04.002
- Author:
Ze-Ying LIU
1
;
Fang YANG
2
;
Wei NIE
1
;
Zhi-Qiang YAN
3
;
Qian-Yun SHI
1
;
Bin YUAN
4
;
Li-Rong LIU
1
Author Information
1. Department of Clinical Hematology， School of Medical Laboratory Science Guizhou Medical University, Guiyang 550004, Guizhou Province, China.
2. Department of Clinical Hematology， School of Medical Laboratory Science Guizhou Medical University, Guiyang 550004, Guizhou Province, China,Department of Laboratory Medicine, Guizhou Cancer Hospital, Guiyang 550001, Guizhou Province, China,E-mail: 465920538@qq.com.
3. Department of Gastrointestinal Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China.
4. Department of Hematology, Guizhou Cancer Hospital, Guiyang 550001, Guizhou Province, China.
- Publication Type:Journal Article
- MeSH:
Alternative Splicing;
HL-60 Cells;
Humans;
Leukemia/genetics*;
Membrane Proteins/genetics*;
Mitochondrial Proteins/genetics*;
RNA Splicing;
U937 Cells;
beta-Lactamases/genetics*
- From:
Journal of Experimental Hematology
2021;29(4):1019-1027
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression of different transcripts of lactamase β（LACTB) gene in leukemic cell lines.
METHODS:NCBI website and DNAstar software were used to detect the Bioinformatics analysis of LACTB. The expression of different transcripts of LACTB gene in leukemic cell lines (THP-1, HL60, K562, U937, Jurkat and Raji) was detected by reverse transcription PCR (RT-PCR), DNA and clone sequencing; the expression of different transcripts of LACTB gene in leukemic cell lines was detected by Quantitative Real-time PCR.
RESULTS:There were a variety of splicing isomers in LACTB, and it could produce a variety of protein isomers with conserved N-terminal and different C-terminal, moreover, there were many splice isoforms of LACTB in leukemia cell lines, and there were different expression patterns in different cell lines, including XR1, V1, V2 and V3. The expression of total LACTB showed high in HL60 cells, while low in Raji cells, and the difference was statistically significant (P<0.05). The V1 was high expression in U937 cells but low in Raji cells, and the difference was statistically significant (P<0.05). V2 was high expression in HL60 cells but lowly in Raji cells, and the difference was statistically significant (P<0.05). The expression of V3 was low in THP-1 cells, which was significantly different as compared with that in normal bone marrow (P<0.05).
CONCLUSION:The reaserch found that there are many splice isomers of LACTB in leukemic cell lines, and there are different expression patterns in different cell lines.
