Serological and molecular studies of a rare A subgroup.
10.3760/cma.j.cn511374-20200911-00660
- Author:
Bin HAN
1
;
Yuli ZHU
;
Yahui QU
;
Li LIU
;
Zhihui FENG
Author Information
1. Qingdao Blood Center, Qingdao, Shandong 266071, China. qd_hla@163.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System/genetics*;
Alleles;
Exons;
Genotype;
Humans;
Phenotype
- From:
Chinese Journal of Medical Genetics
2021;38(8):807-808
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the genotype of an individual suspected for Aw through DNA sequencing.
METHODS:Serologic testing was carried out with standard methods. Exons 6 and 7 of the ABO genes were amplified by PCR and subjected to direct sequencing or sequenced after gene cloning.
RESULTS:Serological testing showed that the forward typing and reverse typing were Aw and A, respectively. DNA sequencing revealed that the individual has carried an Aw allele and an O allele. Haplotype sequencing of each allele has revealed a nt543 variant (543G>C) in the Aw allele.
CONCLUSION:The individual was verified as a rare A subtype, which was previously unreported in mainland China.
