Screening of interacting proteins of idiopathic gonadotropin-releasing hormone deficiency pathogenic gene RNF216.
10.3760/cma.j.cn511374-20200731-00569
- Author:
Wenting DAI
1
;
Zuiming JIANG
;
Min GU
;
Yong ZHU
;
Manling TANG
;
Xiang CHEN
Author Information
1. Center of Clinical Laboratory, the Affiliated Zhuzhou Hospital of Xiangya Medical College, Central South University, Zhuzhou, Hunan 412007, China. 152501019@csu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Gene Library;
Gonadotropin-Releasing Hormone/genetics*;
Humans;
Proteins;
Two-Hybrid System Techniques;
Ubiquitin-Protein Ligases/genetics*
- From:
Chinese Journal of Medical Genetics
2021;38(7):631-634
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen proteins interacting with ring finger protein 216(RNF216) through yeast two hybrid experiment, and further clarify the role of RNF216 in the pathogenesis of gonadotropin-releasing hormone deficiency.
METHODS:A recombinant expression vector pGBKT7-RNF216 was constructed and transformed into yeast Y2HGold, which was hybridized with a human cDNA library in order to screen proteins interacting with RNF216. The interaction was verified in yeast Y2HGold.
RESULTS:A recombinant expression vector pGBKT7-RNF216 was successfully constructed and expressed in yeast Y2HGold. Filamin B (FLNB) was identified by yeast two hybrid experiment, and their interaction was verified in yeast Y2HGold.
CONCLUSION:An interaction between FLNB and RNF216 was identified through yeast two hybrid experiment. RNF216 may affect the proliferation and migration of GnRH neurons by regulating FLNB or FLNB/FLNA heterodimers.
