Research on in vivo metabolites of Longxue Tongluo Capsules by liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectrometry.
10.19540/j.cnki.cjcmm.20210512.201
- Author:
Jing SUN
1
;
Jia-Ni LIU
2
;
Yue-Ting LI
3
;
Hui-Xia HUO
3
;
Xia XU
3
;
Hui XIA
3
;
Yue-Lin SONG
3
;
Qing-Ying ZHANG
4
;
Peng-Fei TU
5
;
Jun LI
3
Author Information
1. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China Laboratory of Quality & Safety Risk Assessment on Agro-products Processing of the Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Product Quality and Safety Management and Control of the Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences Beijing 100193,China.
2. Laboratory of Quality & Safety Risk Assessment on Agro-products Processing of the Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Product Quality and Safety Management and Control of the Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences Beijing 100193,China.
3. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
4. State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center Beijing 100191,China.
5. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center Beijing 100191,China.
- Publication Type:Journal Article
- Keywords:
Chinese dragon′s blood;
LC-IT-TOF-MS;
Longxue Tongluo Capsules;
loureirin A;
loureirin C;
metabolite
- MeSH:
Administration, Oral;
Animals;
Capsules;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal;
Gas Chromatography-Mass Spectrometry;
Rats
- From:
China Journal of Chinese Materia Medica
2021;46(18):4841-4848
- CountryChina
- Language:Chinese
-
Abstract:
Longxue Tongluo Capsules(LTC) has good efficacy against blood stasis syndrome during the recovery period of ischemic stroke. Its main active ingredient is the phenolic extract of Chinese dragon's blood. In our previous study, the primary mass fragmentation pathways of phenolic derivatives from LTC were clarified. Herein, the metabolites in rat plasma were characterized following the oral administration of loureirin A and loureirin C using liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectro-metry(LC-IT-TOF-MS), with 18 and 55 metabolites identified, respectively. On this basis, with the help of the obtained accurate molecular weight, characteristic fragment ions, reference comparison, combined with LTC database and natural products database self-created in our group, 18 prototypes and 106 metabolites were tentatively identified in rat plasma after oral gavage of LTC at a dose of 500 mg·kg~(-1). Glucuronidation, sulfonation, and methylation were major biotransformation pathways of LTC. This study preliminarily clarified the LTC constituents absorbed into blood and laid the foundation for clarifying the effective substances of LTC.
