Content changes of triterpene saponins in crude and sweated Dipsacus asper:an iTRAQ-based analysis.
10.19540/j.cnki.cjcmm.20210311.303
- Author:
Hua HE
1
;
Jiao XU
1
;
Tao ZHOU
1
;
Tai-Min ZHOU
1
;
Juan GUO
2
;
Wei-Ke JIANG
1
;
Cheng-Hong XIAO
1
;
An-Hui GONG
1
Author Information
1. Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
2. National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Dipsacus asper;
differential protein;
isobaric tags for relative and absolute quantification(iTRAQ);
sweating;
triterpene saponins
- MeSH:
Chromatography, Liquid;
Dipsacaceae;
Humans;
Saponins;
Sweating;
Tandem Mass Spectrometry;
Triterpenes
- From:
China Journal of Chinese Materia Medica
2021;46(18):4730-4735
- CountryChina
- Language:Chinese
-
Abstract:
The present study aimed to explore the mechanism of the sweating of Dipsacus asper on content changes of triterpene sa-ponins by detecting the total triterpene saponins and the index component asperosaponin Ⅵ in the crude and sweated D. asper, and analyzing the differentially expressed proteins by isobaric tags for relative and absolute quantification(iTRAQ) combined with LC-MS/MS. After sweating, the content of total triterpene saponins decreased manifestly, while that of asperosaponin Ⅵ increased significantly. As revealed by the iTRAQ-LC-MS/MS analysis, 140 proteins with significant differential expression were figured out, with 50 up-regulated and 90 down-regulated. GO analysis indicated a variety of hydrolases, oxido-reductases, and transferases in the differential proteins. The results of activity test on two differentially expressed oxido-reductases were consistent with those of the iTRAQ-LC-MS/MS analysis. As demonstrated by the analysis of enzymes related to the triterpene saponin biosynthesis pathway, two enzymes(from CYP450 and UGT families, respectively, which are involved in the structural modification of triterpene saponins) were significantly down-regulated after sweating. The findings suggested that sweating of D. asper presumedly regulated triterpene saponins by affecting the expression of downstream CYP450 s and UGTs in the biosynthesis pathway of triterpene saponins of D. asper.
