Simultaneous determination of six pyrrolizidine alkaloids in different parts of Emilia sonchifolia by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20210623.202
- Author:
Ke ZAN
1
;
Ying ZHOU
2
;
Yao-Lei LI
1
;
Ying WANG
1
;
Li-Na LIU
1
;
Hong-Yu JIN
1
;
Tian-Tian ZUO
1
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control Beijing 100050, China.
2. NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Zhejiang Institute for Food and Drug Control Hangzhou 310052, China.
- Publication Type:Journal Article
- Keywords:
Emilia sonchifolia;
UPLC-MS/MS;
content determination;
different part;
pyrrolizidine alkaloids
- MeSH:
Asteraceae;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Pyrrolizidine Alkaloids;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(17):4456-4461
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to develop a UPLC-MS/MS method for simultaneous determination of six pyrrolizidine alkaloids(PAs)--intermedine N-oxide(ImNO), lycopsamine N-oxide(LyNO), seneciphylline(Sp), seneciphylline N-oxide(SpNO), senecionine N-oxide(SnNO), and senkirkine(Sk) in different parts of Emilia sonchifolia. UPLC conditions are as follows: ACQUITY UPLC HSS T3 column(2.1 mm×100 mm, 1.8 μm), mobile phase consisting of 0.05% formic acid and 2.5 mmol·L~(-1) ammonium formate in water(A)-0.05% formic acid and 2.5 mmol·L~(-1) ammonium formate in acetonitrile(B) for gradient elution. MS conditions are as below: electrospray ionization(ESI) in the positive ion mode, multiple reaction monitoring(MRM), and the content of the six PAs was calculated with the external standard method. The results suggested the differences in the six PAs among different parts of E. sonchifolia. Sk was detected in all the four parts, with similar content. SnNO also existed in all the four parts, but the content in roots was significantly higher than that in other parts. Sp and SpNO were found in both roots and flowers, with the content higher in the former than in the later. ImNO and LyNO were only found in leaves, and the content was low. Among the six components detected, ImNO, LyNO, and SpNO were found and determined for the first time, which enriched the toxic components and laid a scientific basis for the quality and safety evaluation of E. sonchifolia.
