Chemical constituents from Clausena excavata and their inhibitory activities against proliferation of synoviocytes.
10.19540/j.cnki.cjcmm.20210429.203
- Author:
Jia-Ming GUO
1
;
Xiao-Mei YU
1
;
Bo JIANG
1
;
Qin-Ting SU
1
;
Wen-Qing CAO
1
;
Yan-Ping LIU
2
;
Yan-Hui FU
1
Author Information
1. Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province, Hainan Normal University Haikou 571158, China.
2. Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province, Hainan Normal University Haikou 571158, China Key Laboratory of Research and Development of Tropical Fruits and Vegetables of Haikou City, Hainan Normal University Haikou 571158, China.
- Publication Type:Journal Article
- Keywords:
Clausena;
Clausena excavata;
alkaloid;
anti-rheumatoid arthritis activity;
coumarin;
lignan
- MeSH:
Cell Proliferation;
Chromatography, Reverse-Phase;
Clausena;
Plant Leaves;
Synoviocytes
- From:
China Journal of Chinese Materia Medica
2021;46(17):4438-4445
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents from the stems and leaves of Clausena excavata were isolated and purified by column chromatography with silica gel, ODS, Sephadex LH-20 and RP-HPLC. The chemical structures of the isolated compounds were identified on the basis of physicochemical properties, spectroscopic analysis, as well as the comparisons with the data reported in literature. Nineteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C. excavata, which were identified as methyl orsellinate(1), syringaresinol(2), lenisin A(3), scopoletin(4), osthenol(5), N-benzoyltyrarnine methyl ether(6), N-p-coumaroyltyramine(7), aurantiamide acetate(8), 1H-indole-3-carboxaldehyde(9), furostifoline(10), clausenalansine E(11), 3-formylcarbazole(12), clausine L(13), clausine E(14), methyl carbazole-3-carboxylate(15), glycosinin(16), murrayafoline A(17), clausine H(18) and 2,7-dihydroxy-3-formyl-1-(3'-methyl-2'-butenyl)carbazole(19). Among these isolated compounds, compounds 1-11 were isolated from C. excavata for the first time, and compounds 1, 2 and 10 were isolated from the genus Clausena for the first time. In addition, this study evaluated the anti-rheumatoid arthritis activities of compounds 1-19 by measuring their anti-proliferative effects on synoviocytes in vitro according to MTS method. Compounds 10-19 displayed remarkable anti-rheumatoid arthritis activities, which exhibited the inhibitory effects on the proliferation of MH7 A synovial fibroblast cells with the IC_(50) values ranging from(27.63±0.18) to(235.67±2.16) μmol·L~(-1).
