Mechanism of Sanhuang Decoction in alleviating dextran sulfate sodium induced ulcerative colitis in mice with Candida albicans colonization:based on high-throughput transcriptome sequencing.
10.19540/j.cnki.cjcmm.20210521.701
- Author:
Ke-Long MA
1
;
Zhi-Jun HAN
2
;
Juan SUN
3
;
Xiao-Fen TAN
2
;
Tian-Ming WANG
3
;
Jing SHAO
3
;
Gui-Ming YAN
3
;
Chang-Zhong WANG
3
Author Information
1. College of Integrated Chinese and Western Medicine/College of Life Science,Anhui University of Chinese Medicine Hefei 230012,China Institute of Integrated Chinese and Western Medicine,Anhui Academy of Chinese Medicine Hefei 230012,China Anhui Province Key Laboratory of Traditional Chinese Medicinal Decoction Pieces of New Manufacturing Technology,Anhui University of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'An Medicine,Ministry of Education,Anhui Academy of Chinese Medicine Hefei 230012,China.
2. College of Integrated Chinese and Western Medicine/College of Life Science,Anhui University of Chinese Medicine Hefei 230012,China.
3. College of Integrated Chinese and Western Medicine/College of Life Science,Anhui University of Chinese Medicine Hefei 230012,China Institute of Integrated Chinese and Western Medicine,Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'An Medicine,Ministry of Education,Anhui Academy of Chinese Medicine Hefei 230012,China.
- Publication Type:Journal Article
- Keywords:
Candida albicans;
NOD-like receptor signaling pathway;
Sanhuang Decoction;
transcriptome sequencing;
ulcerative colitis
- MeSH:
Animals;
Candida albicans/genetics*;
Colitis, Ulcerative/genetics*;
Colon;
Dextran Sulfate/toxicity*;
Disease Models, Animal;
Drugs, Chinese Herbal;
High-Throughput Nucleotide Sequencing;
Mice;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2021;46(15):3915-3925
- CountryChina
- Language:Chinese
-
Abstract:
This study explored the mechanism of Sanhuang Decoction(SHD) in treating dextran sulfate sodium(DSS)-induced ulcerative colitis(UC) in mice with Candida albicans(Ca) colonization via high-throughput transcriptome sequencing. Specifically, the animal model was established by oral administration of 3.0% DSS for 7 days followed by intragastrical administration of Ca suspension at 1.0 × 10~8 cells for 4 days and then the mice were treated with SHD enema for 7 days. Afterwards, the general signs were observed and the disease activity index(DAI) was recorded every day. After mice were sacrificed, colon length and colon mucosa damage index(CMDI) were determined and the histomorphology was observed with the HE staining method. The fungal loads of feces were detected with the plate method. Anti-saccharomyces cerevisiae antibody(ASCA) and β-1,3-glucan in serum, and TNF-α, IL-1β, and IL-6 in serum and colon were detected by ELISA. High-throughput RNA sequencing method was adopted to identify transcriptome of colon tissues from the control, model and SHD(15.0 g·kg~(-1)) groups. Differentially expressed genes(DEGs) among groups were screened and the GO and KEGG pathway enrichment analysis of the DEGs was performed. The expression levels of NLRP3, ASC, caspase-1, and IL-1β genes related to the NOD-like receptor signaling pathway which involved 9 DEGs, were examined by qRT-PCR and Western blot. The results demonstrated that SHD improved the general signs, decreased DAI and Ca loads of feaces, alleviated colon edema, erosion, and shortening, and lowered the content of β-1,3-glucan in serum and TNF-α, IL-1β, and IL-6 in serum and colon tissues of mice. Transcriptome sequencing revealed 383 DEGs between SHD and model groups, which were mainly involved in the biological processes of immune system, response to bacterium, and innate immune response. They were mainly enriched in the NOD-like signaling pathway, cytokine-cytokine interaction pathway, and retinol metabolism pathway. Moreover, SHD down-regulated the mRNA and protein levels of NLRP3, caspase-1, and IL-1β. In a word, SHD ameliorates DSS-induced UC in mice colonized with Ca, which probably relates to its regulation of NOD-like receptor signaling pathway.
