Protective effect and mechanism of Wangshi Baochi Pills against acute alcoholic liver/stomach injury in mice.
10.19540/j.cnki.cjcmm.20210408.401
- Author:
Song YE
1
;
Ling-Li REN
2
;
Xi CHEN
2
;
Bing ZHAO
1
;
Yang YANG
3
;
Ling WENG
3
;
Peng CAO
4
;
Juan YE
3
Author Information
1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China.
2. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
3. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China.
4. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
ADH;
Wangshi Baochi Pills;
acute alcoholic liver injury;
antioxidant stress
- MeSH:
Animals;
Chemical and Drug Induced Liver Injury;
Ethanol;
Liver/metabolism*;
Liver Diseases, Alcoholic;
Mice;
Oxidative Stress;
Stomach
- From:
China Journal of Chinese Materia Medica
2021;46(15):3900-3906
- CountryChina
- Language:Chinese
-
Abstract:
As a common disease worldwide, alcoholic liver injury is caused by long-term or excessive intake of alcohol and triggers cell death due to alcohol metabolism and reactive oxygen species(ROS)-mediated cytotoxicity. Wangshi Baochi(WSBC) Pills have been widely adopted in clinical practice for evacuating stasis, resolving turbidity, clearing heat, tranquilizing mind, invigorating sto-mach, promoting digestion, purging fire and removing toxin. This study aimed to investigate the efficacy of WSBC Pills in dispelling the effect of alcohol and protecting against acute alcoholic liver/stomach injury in mice, and preliminarily investigate its possible mole-cular mechanism. The results found that the preventive treatment with WSBC Pills contributed to elevating the activity of alcohol dehydrogenase(ADH) and its expression in liver and shortening the time required for sobering up of mice with acute alcoholic liver injury. The staining of liver pathological sections as well as the detection of serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) and liver ROS levels revealed that WSBC Pills protected the liver by reducing serum AST and ALT. It suppressed oxidative stress-induced liver injury by lowering liver ROS and elevating superoxide dismutase(SOD), and the liver-protecting effect was superior to that of silibinin. Western blot assay confirmed that WSBC Pills inhibited the oxidative stress by up-regulating SOD1 and NAD(P)H: quinone oxidoreductase 1(NQO-1). In addition, WSBC Pills lowered the ROS level to protect against the acute alcoholic stomach injury in mice. The findings have suggested that WSBC Pills alleviated the acute alcoholic liver/stomach injury in mice by increasing ADH and resisting oxidative stress.
