Analysis and evaluation of volatile oil content in leaves of different Artemisia argyi germplasm resources.
10.19540/j.cnki.cjcmm.20210523.102
- Author:
Chang-Jie CHEN
1
;
Dan-Dan LUO
1
;
Yu-Huan MIAO
1
;
Li-Ping KANG
2
;
Lan-Ping GUO
2
;
Da-Hui LIU
1
;
Lu-Qi HUANG
2
Author Information
1. Pharmacy Faculty, Hubei University of Chinese Medicine Wuhan 430065, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Artemisia argyi;
GC-MS;
clustering analysis;
comprehensive evaluation;
germplasm resources;
volatile oil
- MeSH:
Artemisia;
Distillation;
Oils, Volatile;
Plant Breeding;
Plant Leaves
- From:
China Journal of Chinese Materia Medica
2021;46(15):3814-3823
- CountryChina
- Language:Chinese
-
Abstract:
Volatile oil is the main effective component and an important quality indicator of Artemisia argyi leaves. In this study, 100 germplasm resources of A. argyi were collected from all the related habitats in China. The total volatile oils in A. argyi leaves were extracted by steam distillation and the content was determined by GC-MS. The result demonstrated that the content of total volatile oils was in the range of 0.53%-2.55%, with the average of 1.43%. A total of 39 chemical constituents were identified from the volatile oils, including 13 shared by the 100 germplasm resources. Clustering analysis of the 39 constituents showed that the 100 A. argyi samples were categorized into groups Ⅰ(9), Ⅱ(2), Ⅲ(66) and Ⅳ(23), and group Ⅲ had the most volatile medicinal components, with the highest content. Five principal components(PCs) were extracted from 13 shared constituents, which explained 73.454% of the total variance. PC1, PC2, and PC3 mainly reflected the pharmacological activity of volatile oils and the rest two the aroma information. The volatile oils identified in this study lay a foundation for variety breeding of and rational utilization of volatile oils in A. argyi leaves.
